Larger motors like your V6 can benefit from a direct fuel line connection to the fuel pump. Those quick disconnects can restrict fuel flow or allow air into the fuel line. I recommend you get a 3/8" brass hose barb to hose barb and install it with two clamps, replacing the quick connect.
You should remove the anti siphon valve in the permanent fuel tank, for testing. Once you get the motor working, you can re install it.
You should remove the anti siphon valve in the permanent fuel tank, for testing. Once you get the motor working, you can re install it.